KAT provides a suite of tools that, through the use of k-mer counts, help the user address or identify issues such as determining sequencing completeness for assembly, assessing sequencing bias, identifying contaminants, validating genomic assemblies and filtering content. KAT is geared primarily to work with high-coverage genomic reads from Illumina devices, although can work with any fasta or fastq sequence file.
A new solid phase extraction technology has been developed to reduce the time associated with sample preparation methods by eliminating traditional steps in the extraction workflow. Leveraging this technology, a method protocol was developed using a load, wash and elute approach.
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Sample clean-up is performed in the same plate, without the need to transfer samples, saving time and reducing risky sample handling steps. This method provides high analyte recovery, reduced extraction time due to the elimination of a sample transfer step as well as the elimination of the column conditioning and equilibration steps, and a reduced risk for sample carryover or cross-contamination due to the elimination of the sample transfer step.
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